9FCU
CysG(N-16)-H121A mutant in complex with SAH from Kitasatospora cystarginea
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-06-03 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 100.780, 49.710, 102.510 |
| Unit cell angles | 90.00, 90.08, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.850 |
| R-factor | 0.19336 |
| Rwork | 0.192 |
| R-free | 0.23145 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 1.172 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.950 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.094 | 0.683 |
| Number of reflections | 85811 | 12473 |
| <I/σ(I)> | 9 | 2.6 |
| Completeness [%] | 98.3 | 98.8 |
| Redundancy | 2.9 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.1 M HEPES; 0.02 M MgCl2; 22% Sodium Polyacrylate 5100 |
