9FCF
Medicago truncatula 5'-ProFAR isomerase (HISN3) D57N mutant in complex with ProFAR
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P13 (MX1) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2023-09-26 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9763 |
Spacegroup name | P 4 |
Unit cell lengths | 91.301, 91.301, 35.836 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.280 - 2.360 |
R-factor | 0.2265 |
Rwork | 0.224 |
R-free | 0.25690 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 0.821 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 80.000 | 2.500 |
High resolution limit [Å] | 2.360 | 2.360 |
Rmerge | 0.150 | 0.959 |
Rmeas | 0.160 | 1.024 |
Number of reflections | 12535 | 1978 |
<I/σ(I)> | 10.74 | 2.16 |
Completeness [%] | 99.9 | |
Redundancy | 8.2 | |
CC(1/2) | 0.996 | 0.842 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | 0.2 M sodium acetate, 0.1 M sodium cacodylate pH 6.5, 15% PEG 8000 |