9FCC

HEN EGG-WHITE Lysozyme incubated at 80% relative humidity

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, DESY BEAMLINE P09 HiPhaX
Synchrotron site	PETRA III, DESY
Beamline	P09 HiPhaX
Temperature [K]	298
Detector technology	PIXEL
Collection date	2022-11-12
Detector	DECTRIS EIGER R 4M
Wavelength(s)	0.7749
Spacegroup name	P 43 21 2
Unit cell lengths	79.479, 79.479, 37.886
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.740 - 1.400
R-factor	0.1535
Rwork	0.151
R-free	0.18360
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.972
Data reduction software	XDS
Data scaling software	XDS
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.740	1.450
High resolution limit [Å]	1.400	1.400
Number of reflections	24447	2357
<I/σ(I)>	21.42	2.75
Completeness [%]	99.9
Redundancy	13.1
CC(1/2)	0.999	0.852

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	1M NaCl, 35% (v/v) ethylene glycol, 20% (w/v) PEG4000, 50 mM sodium acetate pH 3.5