9FC5

HEN EGG-WHITE Lysozyme incubated at 99% relative humidity

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, DESY BEAMLINE P09 HiPhaX
Synchrotron site	PETRA III, DESY
Beamline	P09 HiPhaX
Temperature [K]	298
Detector technology	PIXEL
Collection date	2022-11-12
Detector	DECTRIS EIGER R 4M
Wavelength(s)	0.7749
Spacegroup name	P 43 21 2
Unit cell lengths	79.505, 79.505, 37.950
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.250 - 1.400
R-factor	0.1494
Rwork	0.147
R-free	0.17910
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	0.992
Data reduction software	XDS
Data scaling software	XDS
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.250	1.450
High resolution limit [Å]	1.400	1.400
Number of reflections	24530	2398
<I/σ(I)>	20.1	1.2
Completeness [%]	100.0
Redundancy	25.8
CC(1/2)	1.000	0.600

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	3.5	298	1M NaCl, 35% (v/v) ethylene glycol, 20% (w/v) PEG4000, 50 mM sodium acetate pH 3.5