9FBS
Deletion mutant of chitinase MmChi60
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-10-28 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.9184 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 50.764, 74.009, 172.538 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.134 - 2.354 |
| Rwork | 0.186 |
| R-free | 0.26550 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.356 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.500 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.144 | 0.961 |
| Rmeas | 0.152 | 1.015 |
| Number of reflections | 13787 | 2113 |
| <I/σ(I)> | 12.7 | 2.24 |
| Completeness [%] | 99.3 | 96 |
| Redundancy | 9.4 | 9.6 |
| CC(1/2) | 0.997 | 0.762 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 20% (w/v) PEG 4000, 0.4 M MgCl2, 0.1 M Tris pH 8.0 |
