9FBQ

Deletion mutant of chitinase MmChi60

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-11-19
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.9763
Spacegroup name	C 2 2 21
Unit cell lengths	51.114, 72.590, 174.883
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.671 - 1.480
Rwork	0.176
R-free	0.20650
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.676
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.570
High resolution limit [Å]	1.480	1.480
Rmerge	0.080	0.810
Rmeas	0.086	0.877
Number of reflections	54649	8529
<I/σ(I)>	14.17	2.08
Completeness [%]	99.6	98
Redundancy	7.2	6.8
CC(1/2)	0.998	0.635

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	293	45% (v/v) MPD, 0.2 M ammonium acetate, 0.1 M Bis-tris