9FBO

Deletion mutant of chitinase MmChi60

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-11-19
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	.9763
Spacegroup name	H 3
Unit cell lengths	225.739, 225.739, 65.311
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	65.165 - 2.690
Rwork	0.194
R-free	0.25190
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.593
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	66.000	2.840
High resolution limit [Å]	2.680	2.680
Rmerge	0.092	0.855
Rmeas	0.099	0.926
Number of reflections	34425	5271
<I/σ(I)>	14.1	1.45
Completeness [%]	98.8	93.2
Redundancy	7	6.4
CC(1/2)	0.999	0.929

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	10% (w/v) PEG 20000, 20% (v/v) PEG MME 550, a mix of monosaccharides: 20 mM of each: D-glucose, D-mannose, D-galactose, L-fucose, D-xylose, N-acetyl-D-glucosamine and 0.1 M MES/imidazole