9FAE
Human adenovirus type 36 fiber knob in complex with 4-O-acetyl-3'-sialyllactose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-05-10 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.129, 98.644, 99.826 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.507 - 1.574 |
| R-factor | 0.156703663775 |
| Rwork | 0.155 |
| R-free | 0.18702 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.240 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.670 |
| High resolution limit [Å] | 1.570 | 1.570 |
| Number of reflections | 133755 | 20662 |
| <I/σ(I)> | 14 | |
| Completeness [%] | 98.8 | 94.3 |
| Redundancy | 6.8 | 6.3 |
| CC(1/2) | 0.999 | 0.651 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 277 | 25% PEG 3350, 185 mM NH4Ac, 0.1 M Bis-Tris pH 5.5 |
