9FA0

The structure of ScoC, a global regulator protein from Geobacillus kaustophilus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-10
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.96
Spacegroup name	I 41 2 2
Unit cell lengths	95.182, 95.182, 156.018
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.590 - 3.150
R-factor	0.2349
Rwork	0.234
R-free	0.25000
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.291
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.590	3.260
High resolution limit [Å]	3.150	3.150
Rmerge	0.069	0.920
Rmeas	0.070	0.960
Rpim	0.020	0.250
Number of reflections	6497	1143
<I/σ(I)>	24.6	2.9
Completeness [%]	99.7
Redundancy	13.1
CC(1/2)	1.000	0.840

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	20% PEG 3350 and 8% Tacsimate pH 4