9F9B

Laser excitation effects on BR: reprocessed dark dataset recorded from Nango et al.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	FREE ELECTRON LASER
Source details	SACLA BEAMLINE BL3
Synchrotron site	SACLA
Beamline	BL3
Temperature [K]	293
Detector technology	CCD
Collection date	2016-02-04
Detector	MPCCD
Wavelength(s)	1.63
Spacegroup name	P 63
Unit cell lengths	62.580, 62.580, 111.810
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	21.920 - 2.000
R-factor	0.1475
Rwork	0.143
R-free	0.18470
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.075
Data reduction software	CrystFEL (0.9.1)
Data scaling software	CrystFEL (0.9.1)
Refinement software	PHENIX ((1.20_4487))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.900	2.100
High resolution limit [Å]	2.000	2.000
Number of reflections	17068	1705
<I/σ(I)>	9.75	1.26
Completeness [%]	100.0
Redundancy	783

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE		294	100 mM Na/K Phosphate buffer pH 5.4 30 % PEG 2000