9F91

Crystal structure of a designed Respiratory Syncytial Virus immunogen in complex with RSV90 fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-08-31
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00
Spacegroup name	P 21 21 2
Unit cell lengths	113.390, 137.650, 87.470
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	73.830 - 2.430
R-factor	0.2526
Rwork	0.251
R-free	0.28660
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.017
RMSD bond angle	0.753
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	73.830	2.517
High resolution limit [Å]	2.430	2.430
Number of reflections	52204	5153
<I/σ(I)>	9.58
Completeness [%]	99.8
Redundancy	6.36
CC(1/2)	0.997	0.580

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291.15	0.1 M MES pH 6.5 and 20 % (v/v) PEG Smear High