9F90
Crystal structure of a designed three-motif Respiratory Syncytial Virus immunogen in complex with motavizumab fab
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-09-30 |
| Detector | DECTRIS EIGER2 S 9M |
| Wavelength(s) | 0.873129 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 184.072, 66.859, 109.410 |
| Unit cell angles | 90.00, 103.87, 90.00 |
Refinement procedure
| Resolution | 61.500 - 2.910 |
| R-factor | 0.2262 |
| Rwork | 0.224 |
| R-free | 0.26220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.081 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 89.350 | 3.014 |
| High resolution limit [Å] | 2.907 | 2.910 |
| Number of reflections | 28710 | 2829 |
| <I/σ(I)> | 6.14 | |
| Completeness [%] | 99.7 | |
| Redundancy | 3.03 | |
| CC(1/2) | 0.989 | 0.738 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291.15 | 0.2 M potassium thiocyanate, 0.1 M sodium cacodylate pH 6.5, and 10 % (v/v) PEG 1000 |
