9F8W
Crystal structure of the apo Pex5 peroxisomal cargo receptor from Trypanosoma brucei
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-11-10 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.9184 |
Spacegroup name | H 3 |
Unit cell lengths | 206.545, 206.545, 67.867 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 47.890 - 2.350 |
R-factor | 0.20025 |
Rwork | 0.198 |
R-free | 0.24605 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.552 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.890 | 2.434 |
High resolution limit [Å] | 2.350 | 2.350 |
Number of reflections | 44917 | 4490 |
<I/σ(I)> | 14.68 | |
Completeness [%] | 99.9 | |
Redundancy | 5.8 | 6 |
CC(1/2) | 0.999 | 0.520 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 293 | 0.9 M sodium-potassium tartrate tetrahydrate, 20% w/v glycerol, 0.05 M Hepes pH 7.4 after 8 days at 293 K |