9F8V

Structure of the mouse 8-oxoguanine DNA Glycosylase mOGG1 in complex with ligand TH7420

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-10-18
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9762
Spacegroup name	P 21 21 21
Unit cell lengths	81.501, 81.598, 170.355
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	58.956 - 2.400
Rwork	0.229
R-free	0.28130
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.432
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0411)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	58.956	2.480
High resolution limit [Å]	2.400	2.400
Number of reflections	45246	4343
<I/σ(I)>	16.9
Completeness [%]	100.0
Redundancy	13.3
CC(1/2)	0.999	0.774

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		294	0.12 M Monosaccharides, 0.1 M bicine/Trizma base pH 8.5, 50 % GOL_P4K