9F8T

Clathrin terminal domain complexed with C-terminus of AAK1L

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-01-22
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9763
Spacegroup name	C 2 2 21
Unit cell lengths	107.539, 130.860, 77.050
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.264 - 1.711
Rwork	0.172
R-free	0.20920
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.677
Data reduction software	MOSFLM (7.2.1)
Data scaling software	Aimless (0.3.11 : 19/08/14)
Phasing software	PHASER (2.6.1)
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.300	1.770
High resolution limit [Å]	1.710	1.710
Rmerge	0.069	0.452
Rmeas	0.097	0.640
Rpim	0.069	0.452
Number of reflections	56178	5147
<I/σ(I)>	4.83	1.15
Completeness [%]	95.5	88.8
Redundancy	1.87	1.83
CC(1/2)	0.987	0.603

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293.15	20% PEG 3,350; 100 mM Bis-Tris propane, pH 6.0; 200 mM sodium citrate; 10 mM DTT