9F8F
SLPL/SLPH (H/L) complex C. difficile Opt2472 strain (SLCT2)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-05-04 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.7085 |
| Spacegroup name | P 1 |
| Unit cell lengths | 75.324, 76.480, 78.229 |
| Unit cell angles | 81.80, 78.86, 66.78 |
Refinement procedure
| Resolution | 35.780 - 3.070 |
| R-factor | 0.2335 |
| Rwork | 0.231 |
| R-free | 0.28310 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.497 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.780 | 3.180 |
| High resolution limit [Å] | 3.070 | 3.070 |
| Number of reflections | 29132 | 2912 |
| <I/σ(I)> | 6.2 | 3.4 |
| Completeness [%] | 98.9 | 99.3 |
| Redundancy | 6.2 | 6.8 |
| CC(1/2) | 0.813 | 0.417 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 1600 mM Sodium citrate tribasic |
