9F8E
SLPL/SLPH (H/L) complex from C. difficile SlpA (Ox247_delta_orf2 strain, SLCT11)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-05-04 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.70846 |
| Spacegroup name | P 1 |
| Unit cell lengths | 47.612, 73.374, 90.799 |
| Unit cell angles | 91.64, 95.74, 96.11 |
Refinement procedure
| Resolution | 39.080 - 3.100 |
| R-factor | 0.1813 |
| Rwork | 0.178 |
| R-free | 0.23440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.582 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.080 | 3.210 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmeas | 0.430 | |
| Number of reflections | 21675 | 2166 |
| <I/σ(I)> | 7.5 | 4.3 |
| Completeness [%] | 96.8 | 93.2 |
| Redundancy | 6.7 | 6.6 |
| CC(1/2) | 0.407 | 0.159 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293.15 | 4mM Oxometalates, 0.05M Tris (base), 0.o5M BICINE, 20% v/v Glycerol; 10% w/v PEG 4000 |
