9F7Z
Crystal structure of human 4-1BB/TNFRSF9 in complex with the anti-4-1BB DARPin protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-07-20 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.999953 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 170.330, 102.469, 167.366 |
| Unit cell angles | 90.00, 91.54, 90.00 |
Refinement procedure
| Resolution | 167.310 - 2.810 |
| R-factor | 0.2436 |
| Rwork | 0.241 |
| R-free | 0.29120 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.550 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 167.310 | 3.173 |
| High resolution limit [Å] | 2.810 | 2.815 |
| Rmerge | 0.157 | 1.042 |
| Number of reflections | 47018 | 2352 |
| <I/σ(I)> | 7.1 | 1.5 |
| Completeness [%] | 92.4 | 60.1 |
| Redundancy | 4.3 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.10 M Citric acid pH=5.00 1.60 M (NH4)2SO4 |
