9F7M

Blastococcus Orn bound to pGG

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, DESY BEAMLINE P11
Synchrotron site	PETRA III, DESY
Beamline	P11
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-09-01
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	1.0329
Spacegroup name	P 1 21 1
Unit cell lengths	79.570, 117.701, 99.466
Unit cell angles	90.00, 93.62, 90.00

Refinement procedure

Resolution	47.280 - 1.650
R-factor	0.1708
Rwork	0.171
R-free	0.19100
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.903
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.280	1.709
High resolution limit [Å]	1.650	1.650
Number of reflections	216829	21705
<I/σ(I)>	11.42
Completeness [%]	99.0
Redundancy	6.8
CC(1/2)	0.997	0.795

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	292	0.2 M Sodium chloride, 0.1 M BIS-TRIS (pH 5.5), 25%(w/v) PEG3350