9F7D

Rhodococcus diDNase bound to deoxy-pGG

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, DESY BEAMLINE P11
Synchrotron site	PETRA III, DESY
Beamline	P11
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-12-12
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	1.033220
Spacegroup name	C 2 2 21
Unit cell lengths	51.837, 61.298, 135.109
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.580 - 2.000
R-factor	0.1879
Rwork	0.183
R-free	0.22900
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.042
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.580	2.071
High resolution limit [Å]	2.000	2.000
Rmerge	0.159	1.389
Rpim	0.045	0.393
Number of reflections	14916	1457
<I/σ(I)>	14
Completeness [%]	99.8
Redundancy	13.2
CC(1/2)	0.998	0.865

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	292	0.2 M ammonium chloride, 20%(w/v) PEG3350