9F7C

SARS-CoV-2 Nucleocapsid N-terminal domain (NTD) mutant S105I

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-02-05
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 41 21 2
Unit cell lengths	58.616, 58.616, 111.588
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.450 - 2.000
Rwork	0.241
R-free	0.27330
Structure solution method	SAD
RMSD bond length	0.011
RMSD bond angle	2.561
Data reduction software	autoPROC (1.1.7)
Data scaling software	Aimless (0.7.13)
Phasing software	CRANK2 (2.0.339)
Refinement software	REFMAC (5.8.0430 (refmacat 0.4.88))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.450	2.050
High resolution limit [Å]	2.000	2.000
Number of reflections	13840	921
<I/σ(I)>	15.8	1.2
Completeness [%]	99.3
Redundancy	12.4
CC(1/2)	0.999	0.938

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	22% PEG 3350 200mM Zinc Acetate dihydrate