9F7A
SARS-CoV-2 Nucleocapsid N-terminal domain (NTD) mutant P80R
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
| Synchrotron site | PETRA III, EMBL c/o DESY |
| Beamline | P13 (MX1) |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-04-30 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97626 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 66.753, 66.753, 121.214 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 57.810 - 1.900 |
| Rwork | 0.277 |
| R-free | 0.34770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.782 |
| Data reduction software | autoPROC (1.1.7) |
| Data scaling software | Aimless (0.7.13) |
| Phasing software | PHASER (2.8.3) |
| Refinement software | REFMAC (5.8.0430 (refmacat 0.4.88)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 121.210 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 13269 | 815 |
| <I/σ(I)> | 20.2 | 0.3 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 36.4 | |
| CC(1/2) | 1.000 | 0.380 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9 | 285 | 22% PEG 3350 200mM Zinc Acetate dihydrate |
