9F49

Structure of the bacteriophage K gp155 C-terminal domain, seleno-methionine modified version

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-09-29
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97872
Spacegroup name	P 21 21 21
Unit cell lengths	42.397, 42.861, 123.646
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	61.823 - 1.621
Rwork	0.158
R-free	0.18370
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	1.319
Data reduction software	XDS
Data scaling software	Aimless (0.6.2)
Phasing software	CRANK2 (2.0.281)
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	123.650	1.710
High resolution limit [Å]	1.620	1.620
Rmerge	0.089	0.471
Rmeas	0.093	0.529
Rpim	0.027	0.232
Number of reflections	26390	2360
<I/σ(I)>	20.9	3.2
Completeness [%]	89.4	56.1
Redundancy	10.7	5
CC(1/2)	0.998	0.729

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	294	20% (w/v)PEG 1500, 100 mM HEPES-NaOH pH 7.5, 10 mM Tris-HCl