9F3M

Crystal structure of Kluyveromyces lactis glucokinase mutant H304Q in complex with fructose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-03-16
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.9184
Spacegroup name	I 4
Unit cell lengths	207.116, 207.116, 89.376
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	51.780 - 2.600
R-factor	0.2478
Rwork	0.247
R-free	0.25840
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.002
RMSD bond angle	0.488
Data reduction software	DIALS (1.dev.877-g8f078f0)
Data scaling software	Aimless (0.5.31)
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	73.230	2.670
High resolution limit [Å]	2.600	2.600
Number of reflections	58352	4493
<I/σ(I)>	7.9	0.2
Completeness [%]	100.0	100
Redundancy	13	13.1
CC(1/2)	0.997	0.349

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	droplet: 1:1 mixture of protein and reservoir protein supplemented with: 9.8 mM fructose 9.8 mM DTT reservoir solution: 1.4 M sodium malonate pH 6.3