9F3H
Undecorated 13pf mosaic 20%E254Q - 80% E254QN microtubule from recombinant human tubulin
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | TFS GLACIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 200 |
| Detector | FEI FALCON IV (4k x 4k) |
3D Reconstruction
| Method | HELICAL |
| Resolution (Å) | 4.3 |
| Number of Particle | 44726 |
| Software | RELION,PHENIX,Coot |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
