9F2T
Ferric-mycobactin receptor (FemA)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-02-20 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.95374 |
| Spacegroup name | P 1 |
| Unit cell lengths | 83.678, 84.954, 86.702 |
| Unit cell angles | 89.97, 61.60, 66.26 |
Refinement procedure
| Resolution | 75.550 - 2.200 |
| R-factor | 0.2274 |
| Rwork | 0.225 |
| R-free | 0.27060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.919 |
| Data reduction software | xia2 |
| Data scaling software | DIALS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 75.550 | 2.080 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.201 | 1.330 |
| Number of reflections | 115401 | 5668 |
| <I/σ(I)> | 7.1 | |
| Completeness [%] | 98.2 | |
| Redundancy | 3.6 | |
| CC(1/2) | 0.987 | 0.325 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | PEG 400, calcium acetate, sodium chloride |
