9F2M
To be published
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2021-03-11 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54789 |
| Spacegroup name | P 32 |
| Unit cell lengths | 36.230, 36.230, 69.590 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.600 - 1.750 |
| R-factor | 0.1945 |
| Rwork | 0.192 |
| R-free | 0.24020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.364 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.1_5286) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.850 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmeas | 0.037 | 0.224 |
| Number of reflections | 10224 | 1643 |
| <I/σ(I)> | 23.1 | 5.4 |
| Completeness [%] | 98.5 | 99 |
| Redundancy | 3.4 | 3.3 |
| CC(1/2) | 0.999 | 0.964 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 25% (w/v) PEG 1500, 100 mM PCTP (sodium propionic acid, sodium cacodylate, Bis-Tris propane) pH 5.0 |
