9F2K
Myo-inositol-1-phosphate synthase from Thermochaetoides thermophila in complex with NAD
EM specimen
Vitrification | Yes |
Instrument | FEI VITROBOT MARK IV |
EM Data Collection
Microscope | TFS GLACIOS |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 200 |
Cs (mm) | 2.7 |
Detector | FEI FALCON III (4k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 2.48 |
Number of Particle | 309043 |
Software | cryoSPARC,PHENIX,UCSF ChimeraX,Coot,EPU |
Symmetry | D2 |
CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |