9F2D

KIR2DL1 bound to RIFIN RBK21

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-03-03
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	0.97625
Spacegroup name	P 21 21 21
Unit cell lengths	93.399, 99.047, 321.130
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	57.370 - 2.886
R-factor	0.2577
Rwork	0.256
R-free	0.28640
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.850
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	BUSTER (2.10.4 (26-JUL-2023))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	160.570	3.150
High resolution limit [Å]	2.880	2.880
Rpim	0.013	0.817
Number of reflections	53588	2679
<I/σ(I)>	7
Completeness [%]	94.7
Redundancy	6.4
CC(1/2)	0.994	0.643

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	0.1 M Tris-Bicine pH 8.5, 0.1M Amino acid mix, 37.5 % v/v precipitant mix 4