9F2B
Focused refinement of SV2B luminal domain and BoNT/A1 complex
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | TFS KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | GATAN K3 BIOQUANTUM (6k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.49 |
| Software | RELION,cryoSPARC,PHENIX,CTFFIND,Coot |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
