9F26

Crystal structure of the PriS_PriL-Rpa2WH ternary complex from P. abyssi

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-07-23
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.978565
Spacegroup name	P 21 21 21
Unit cell lengths	63.815, 101.505, 177.805
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.800 - 3.501
R-factor	0.2356
Rwork	0.234
R-free	0.25550
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.760
Data reduction software	XDS (VERSION Feb 5, 2021 BUILT=20210323)
Data scaling software	XDS (VERSION Feb 5, 2021 BUILT=20210323)
Phasing software	PHASER
Refinement software	BUSTER (2.10.4)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.800	3.590
High resolution limit [Å]	3.500	3.500
Rmeas	0.131	15.624
Rpim	0.036	4.240
Number of reflections	201145	14465
<I/σ(I)>	9.5	0.3
Completeness [%]	99.9	99.5
Redundancy	13.2	13.4
CC(1/2)	1.000	0.200

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.1	291	60% v/vTacsimate 0.1M Bis-Tris prop 7