9F1T
Psychrophilic laccase (multicopper oxidase) from Oenococcus oeni 229 without Histag
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 180 |
| Detector technology | PIXEL |
| Collection date | 2023-03-29 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97926 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.672, 97.865, 96.098 |
| Unit cell angles | 90.00, 91.65, 90.00 |
Refinement procedure
| Resolution | 68.650 - 3.000 |
| R-factor | 0.22139 |
| Rwork | 0.219 |
| R-free | 0.26629 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.132 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 68.650 | 3.180 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.299 | 0.788 |
| Rmeas | 0.336 | 0.889 |
| Rpim | 0.150 | 0.401 |
| Total number of observations | 14384 | |
| Number of reflections | 19139 | 3066 |
| <I/σ(I)> | 4.4 | 2.3 |
| Completeness [%] | 97.8 | |
| Redundancy | 4.7 | 4.7 |
| CC(1/2) | 0.912 | 0.660 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 294 | 18% PEG 10000, 20% glycerol, 100 mM Tris-HCl pH 8.5 and 100 mM NaCl |
