Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-3 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-3 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-11-16 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 0.9677 |
| Spacegroup name | P 21 2 21 |
| Unit cell lengths | 55.833, 73.835, 201.053 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 59.510 - 2.750 |
| R-factor | 0.2229 |
| Rwork | 0.218 |
| R-free | 0.26610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.091 |
| Data reduction software | xia2 (3.16.0) |
| Data scaling software | STARANISO (3.350) |
| Phasing software | PHASER (2.8.3) |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.520 | 3.230 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Rmerge | 0.166 | 1.536 |
| Number of reflections | 15438 | 2202 |
| <I/σ(I)> | 9.6 | 1.9 |
| Completeness [%] | 91.9 | 69 |
| Redundancy | 13.9 | 13.9 |
| CC(1/2) | 0.998 | 0.730 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 3 | VAPOR DIFFUSION, SITTING DROP | 293 | 88 mM NaOH, 100 mM Bicine, 11% (w/v) PEG 20,000, 1% (v/v) Dioxane, 10 mM Sarcosine (Crystal 3) |
