9F18
Crystal structure of a first-in-class antibody for alpha-1,6-fucosylated prostate-specific antigen
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2021-09-26 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 0.9998 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 72.219, 66.735, 91.713 |
Unit cell angles | 90.00, 112.09, 90.00 |
Refinement procedure
Resolution | 66.010 - 2.520 |
R-factor | 0.1871 |
Rwork | 0.184 |
R-free | 0.25120 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 0.972 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.21_5207+SVN) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 66.010 | 2.800 |
High resolution limit [Å] | 2.520 | 2.520 |
Rmeas | 0.087 | 1.089 |
Number of reflections | 19541 | 978 |
<I/σ(I)> | 15.2 | 1.4 |
Completeness [%] | 90.5 | 50.7 |
Redundancy | 3.1 | 2.9 |
CC(1/2) | 0.999 | 0.503 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 294 | 100 mM potassium thiocyanate and 30% w/v PEG MME 2000 (poly[ethylene glycol] methyl ether |