9EZW

Structure of the single-domain antibody VHH_h3 in complex with human Vsig4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-07-15
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9762
Spacegroup name	P 21 21 21
Unit cell lengths	60.867, 129.941, 144.284
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	64.970 - 1.900
R-factor	0.1942
Rwork	0.190
R-free	0.22800
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.872
Data reduction software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	129.940	2.010
High resolution limit [Å]	1.900	1.900
Number of reflections	89487	14083
<I/σ(I)>	13.61
Completeness [%]	98.3
Redundancy	13.9
CC(1/2)	0.998	0.456

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	294	0,1M Na-citrate pH 5,6 20% 2-propanol 21% PEG 4000