9EZV

Structure of the single-domain antibody VHH_h2 in complex with human Vsig4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-10-14
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9762
Spacegroup name	P 21 21 21
Unit cell lengths	52.291, 53.069, 87.531
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.760 - 1.450
R-factor	0.1755
Rwork	0.174
R-free	0.21510
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.619
Data reduction software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	53.070	1.540
High resolution limit [Å]	1.450	1.450
Rmeas	0.088
Number of reflections	43885	7006
<I/σ(I)>	17.77
Completeness [%]	99.9
Redundancy	13.3
CC(1/2)	1.000	0.423

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	287	0,1M Na-citrate pH 5,6 20% 2-propanol 21% PEG 4000