9EZU

Structure of the single-domain antibody VHH_h1 in complex with human Vsig4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-09-21
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9762
Spacegroup name	P 21 21 21
Unit cell lengths	50.651, 51.404, 89.649
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.710 - 1.050
R-factor	0.1583
Rwork	0.157
R-free	0.17570
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.251
Data reduction software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	51.400	1.110
High resolution limit [Å]	1.050	1.050
Rmeas	0.100	1.020
Number of reflections	109581	17482
<I/σ(I)>	12.4
Completeness [%]	99.9
Redundancy	13.3
CC(1/2)	0.997	0.757

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		287	0,17M ammonium sulfate 15% glycerol 25,5% PEG 4000