9EZL
Crystal structure of trehalose synthase mutant N253H from Deinococcus radiodurans
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL15A1 |
Synchrotron site | NSRRC |
Beamline | BL15A1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-03-01 |
Detector | RAYONIX MX300-HS |
Wavelength(s) | 1.000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 96.208, 196.264, 131.205 |
Unit cell angles | 90.00, 91.66, 90.00 |
Refinement procedure
Resolution | 19.920 - 2.970 |
R-factor | 0.1699 |
Rwork | 0.166 |
R-free | 0.24560 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.104 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 3.050 |
High resolution limit [Å] | 2.970 | 2.970 |
Rmerge | 0.135 | 0.167 |
Number of reflections | 98360 | 93240 |
<I/σ(I)> | 7.5 | |
Completeness [%] | 99.0 | |
Redundancy | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | Tris-HCl pH 8.5, 8% (w/v) polyethylene glycol (PEG) 8000 |