9EZE

Structure of subdomain 3 of the Plasmodium vivx Duffy binding protein (PvDBP) bound to human antibody DB9

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-18
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9999
Spacegroup name	P 21 21 21
Unit cell lengths	76.427, 126.191, 129.863
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	90.500 - 1.550
R-factor	0.2053
Rwork	0.204
R-free	0.22320
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.120
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	BUSTER (2.10.4 (26-JUL-2023))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	90.500	1.605
High resolution limit [Å]	1.550	1.550
Number of reflections	180370	17671
<I/σ(I)>	14.77
Completeness [%]	98.3
Redundancy	6.5
CC(1/2)	0.999	0.580

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2M ammonium acetate, 0.1M sodium acetate pH 4.0, 15% PEG 4000