9EYT

Crystal structure of Yeast Clathrin Heavy Chain N-terminal domain bound to Epsin-1 peptide (LIDL)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	196
Detector technology	PIXEL
Collection date	2022-09-24
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9763
Spacegroup name	P 43 21 2
Unit cell lengths	56.310, 56.310, 254.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	63.731 - 1.740
Rwork	0.185
R-free	0.22990
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.687
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	127.300	127.300	1.770
High resolution limit [Å]	1.740	9.040	1.740
Rmerge	0.068	0.035	2.019
Rmeas	0.071	0.037	2.092
Rpim	0.019	0.010	0.545
Number of reflections	43582	422	2365
<I/σ(I)>	27.2
Completeness [%]	100.0
Redundancy	26	18.4	27.5
CC(1/2)	1.000	0.999	0.843

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		292	0.2M potassium/sodium tartrate 20%(w/v) PEG 3350