9EYP
The structure of octameric pore of RN1 variant of actinoporin Fav
This is a non-PDB format compatible entry.
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK III |
EM Data Collection
| Microscope | TFS GLACIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 200 |
| Cs (mm) | 2.7 |
| Detector | FEI FALCON III (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.05 |
| Number of Particle | 446027 |
| Software | cryoSPARC,Coot,PHENIX,ISOLDE,EPU,UCSF ChimeraX |
| Symmetry | C8 |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
