9EYO
The structure of solubilized octameric pore of actinoporin Fav prepared on POPG, cholesterol, sphingomyelin membranes
This is a non-PDB format compatible entry.
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK III |
EM Data Collection
| Microscope | TFS GLACIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 200 |
| Cs (mm) | 2.7 |
| Detector | FEI FALCON III (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 2.86 |
| Number of Particle | 2705265 |
| Software | cryoSPARC,Coot,PHENIX,ISOLDE,EPU,UCSF ChimeraX |
| Symmetry | C8 |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
