9EYH
Ubiquitin conjugating enzyme Ubc6 UBC domain with isopeptide-linked ubiquitin
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-03-29 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 1 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 105.753, 53.490, 111.798 |
| Unit cell angles | 90.00, 103.15, 90.00 |
Refinement procedure
| Resolution | 48.010 - 2.600 |
| R-factor | 0.2356 |
| Rwork | 0.234 |
| R-free | 0.26560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.506 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.010 | 2.693 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.085 | |
| Number of reflections | 19017 | 1903 |
| <I/σ(I)> | 17.41 | |
| Completeness [%] | 99.7 | 99.53 |
| Redundancy | 12.1 | 11.8 |
| CC(1/2) | 0.999 | 0.945 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | PEG smear, sodium acetate, sodium chloride |
