9EXB

SARS-CoV-2 Nucleocapsid N-terminal domain NTD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-04-06
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9762
Spacegroup name	P 21 21 21
Unit cell lengths	58.916, 93.073, 96.936
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	67.140 - 2.300
R-factor	0.271
Rwork	0.269
R-free	0.32000
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.900
Data reduction software	autoPROC (1.1.17)
Data scaling software	Aimless (0.7.13)
Phasing software	PHASER (2.8.3)
Refinement software	BUSTER (2.10.4)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	96.980	96.980	2.380
High resolution limit [Å]	2.300	8.910	2.300
Rmerge	0.203	0.063	4.069
Rmeas	0.212	0.066	4.252
Rpim	0.062	0.020	1.224
Number of reflections	24362	501	2359
<I/σ(I)>	11.8	39.2	0.9
Completeness [%]	99.7	99.9	100
Redundancy	21	18.3	22
CC(1/2)	0.998	0.998	0.599

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293	20% PEG 6000 100mM MES pH 6.0 10mM Zinc Chloride