9EWH

SARS-CoV-2 Nucleocapsid N-terminal domain (NTD) mutant Y109A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-02-29
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.96862
Spacegroup name	P 1 21 1
Unit cell lengths	58.836, 55.139, 84.088
Unit cell angles	90.00, 95.24, 90.00

Refinement procedure

Resolution	83.740 - 1.930
R-factor	0.2137
Rwork	0.212
R-free	0.25510
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.000
Data reduction software	autoPROC (1.1.7)
Data scaling software	Aimless (0.7.13)
Phasing software	PHASER (2.8.3)
Refinement software	BUSTER (2.10.4)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	83.740	83.740	1.970
High resolution limit [Å]	1.920	9.030	1.920
Rmerge	0.100	0.035	0.605
Rmeas	0.114	0.040	0.687
Rpim	0.054	0.019	0.320
Number of reflections	40470	433	2755
<I/σ(I)>	12.5	33.4	3.1
Completeness [%]	98.9	99.1	99.2
Redundancy	8.2	7.5	8.4
CC(1/2)	0.998	0.999	0.889

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	277	PEG 3350