9EWD

DNA Polymerase Lambda I493K E529D, TMP:At Ca2+ Ground State Ternary Complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-09-09
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9801
Spacegroup name	P 21 21 21
Unit cell lengths	56.400, 62.470, 139.570
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.890 - 2.120
R-factor	0.2037
Rwork	0.202
R-free	0.24490
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.925
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.550	2.180
High resolution limit [Å]	2.120	2.120
Rpim	0.038	0.690
Number of reflections	28562	4503
<I/σ(I)>	16.6	1.6
Completeness [%]	99.8
Redundancy	13.4
CC(1/2)	0.990	0.794

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	277.15	20 mM bicine pH7.5 300 mM Na-K tartrate 20% PEG 20K