9EW6

Binary structure of 14-3-3s and BRAF phosphopeptide (pS365)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-02-19
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	0.873128
Spacegroup name	C 2 2 21
Unit cell lengths	81.783, 96.043, 80.440
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	62.270 - 1.650
R-factor	0.18669
Rwork	0.185
R-free	0.22100
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.295
Data reduction software	autoPROC
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PDB-REDO

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	80.440	1.680
High resolution limit [Å]	1.650	1.650
Number of reflections	38175	1908
<I/σ(I)>	14.4	1.4
Completeness [%]	99.3
Redundancy	13.5
CC(1/2)	0.998	0.694

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	0.095 M HEPES pH=7.1-7.7 0.19 M CaCl2 5% glycerol 24-29% PEG400