9EVU

HEN EGG-WHITE Lysozyme incubated at 99% relative humidity

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, DESY BEAMLINE P09 HiPhaX
Synchrotron site	PETRA III, DESY
Beamline	P09 HiPhaX
Temperature [K]	298
Detector technology	PIXEL
Collection date	2022-11-12
Detector	DECTRIS EIGER R 4M
Wavelength(s)	0.7749
Spacegroup name	P 43 21 2
Unit cell lengths	79.483, 79.483, 37.966
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.100 - 1.400
R-factor	0.1533
Rwork	0.151
R-free	0.17700
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.995
Data reduction software	XDS
Data scaling software	XDS
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.100	1.450
High resolution limit [Å]	1.400	1.400
Number of reflections	24508	2384
<I/σ(I)>	18.74	2.33
Completeness [%]	99.9
Redundancy	12.5
CC(1/2)	0.994	0.780

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	3.5	298	1M NaCl, 35% (v/v) ethylene glycol, 20% (w/v) PEG4000, 50 mM sodium acetate pH 3.5