9EVL

Crystal Structure of human Collagen Hydroxylysine Galactosyltransferase GLT25D1/COLGALT1: Hg2+ soak for experimental phasing

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-03-15
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.7749
Spacegroup name	I 2 3
Unit cell lengths	220.187, 220.187, 220.187
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.240 - 2.800
R-factor	0.204
Rwork	0.202
R-free	0.23890
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.564
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	HKL2Map
Refinement software	PHENIX ((1.20_4459: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.240	2.910
High resolution limit [Å]	2.800	2.800
Rmerge	0.169	3.609
Rpim	0.051	1.093
Number of reflections	84831	4551
<I/σ(I)>	13.2	0.9
Completeness [%]	100.0	100
Redundancy	23.2	23.3
CC(1/2)	0.998	0.599

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	8% poly-ethylene-glycol (PEG) MW 4000, 100 mM 2-(N-morpholino)ethanesulfonic acid (MES)/NaOH, 20% glycerol