9EVK

Crystal Structure of human Collagen Hydroxylysine Galactosyltransferase GLT25D1/COLGALT1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-09-25
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.8856
Spacegroup name	I 2 3
Unit cell lengths	221.907, 221.907, 221.907
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.620 - 3.000
R-factor	0.199
Rwork	0.197
R-free	0.24160
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.645
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.20_4459: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.620	3.130
High resolution limit [Å]	3.000	3.000
Rmerge	0.130	1.047
Rpim	0.101	0.811
Number of reflections	35735	4385
<I/σ(I)>	6.2	1
Completeness [%]	98.4
Redundancy	3.5
CC(1/2)	0.992	0.402

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	8% poly-ethylene-glycol (PEG) MW 4000, 100 mM 2-(N-morpholino)ethanesulfonic acid (MES)/NaOH, 20% glycerol, pH 6.5